Opp transfer spinach seedlings10/2/2023 ![]() ![]() Baker, a commercially important eucalypt grown in short-rotation coppice cultivation and harvested for its foliar 1,8-cineole oil. The influence of micropropagation on growth and coppicing ability of Eucalyptus polybractea.Ī micropropagation protocol was recently developed for Eucalyptus polybractea R.T. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. ![]() Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review provides a detailed overview of the Dendrobium micropropagation literature. Tissue culture is now an established method for the effective propagation of members of this genus. The protocol is simple, rapid, and efficient for large-scale propagation of African violet and the dual routes of regeneration allow for multiple applications of the technology from simple clonal propagation to induction or selection of variants to the production of synthetic seeds.ĭa Silva, Jaime A Teixeira Cardoso, Jean Carlos Dobránszki, Judit Zeng, Songjunĭendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. In this process, plant regeneration occurs via both somatic embryogenesis and shoot organogenesis simultaneously in the explants induced with the growth regulator thidiazuron (TDZ N-phenyl-N'-1,2,3-thidiazol-5-ylurea). The current chapter describes an optimized protocol for micropropagation of African violets using leaf explants obtained from in vitro grown plants. Development of cost-effective protocols with a high rate of multiplication is a crucial requirement for commercial application of micropropagation. Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant, plant growth regulators in the culture medium, and growth conditions. Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Shukla, Mukund Sullivan, J Alan Jain, Shri Mohan Murch, Susan J Saxena, Praveen K Micropropagation of African violet (Saintpaulia ionantha Wendl.). Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana. Conventional micropropagation techniques, however, may be too costly for commercial purposes. AAA) is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation.
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